Journal of Virological Methods
Peste des petits ruminants (PPR), The causal agent is peste des petits ruminants virus (PPRV), which belongs to the genus Morbillivirus in the family Paramyxoviridae. This genus also includes measles virus (MV), canine distemper virus (CDV) and rinderpest virus (RPV). All are closely related viruses with serological cross reactivity. In this study, we have developed a Luciferase Immunoprecipitation System (LIPS) for the rapid detection of antibodies against PPRV in serum samples and for specific differentiation from antibodies against RPV.
The PPR-LIPS was developed for the specific detection of PPR antibody while eliminating the cross-reactivity with like viruses. It is based on the use of the Renilla luciferase protein to which is fused the c-terminal fragment – amino acids 420-525 of the PPRV nucleoprotein.
This fragment was chosen after consideration of in-house antibody mapping studies suggesting its potential to differentiate PPRV from RPV in antibody binding (Bodjo et al., 2013). Serum samples collected from PPR infected sheep and goats, as well as non-infected animals, were tested by this new test and also by commercially available PPR cELISA kits: the ID Vet ID Screen PPR cELISA kit and the BDSL PPR cELISA kit. They are the current assays recommended for use in the 2013 OIE Manual (World Organization for Animal Health, 2013). The PPR VNT, the OIE recommended test for PPR serological diagnosis for trade issues, was used on a small sub-set of the PPR field serum samples where there was no agreement between the cELISA kits and PPR-LIPS. The results obtained indicate that PPR-LIPS is at least as sensitive as the commercially available PPR cELISA kits, requires lower sample volumes (1 l per assay) and shows no cross reactivity between PPR and rinderpest antibodies in serum samples